Description
The pSX2 expression vector is designed to be combined with your chosen Clean GenomeĀ®Ā E. coliĀ host strain to produce the robust ScarabXpressĀ®-2 Protein Expression System.
- A 3,000-fold induction range is achievable, and strong repression of the patented synthetic T5 promotor prior to induction facilitates robust healthy growth of cultures to high density.
- The high strength synthetic T5 promotor in combination with limited plasmid copy number enhances production of recombinant protein product without wasting energy on plasmid replication.
- Tight regulation of the basal level of transcription prior to induction keeps background expression low, key for target genes that are deleterious to the host.
- pSX2 is compatible with all Clean GenomeĀ® E. coli strains.
- If pSX2 is used with strains other than Clean GenomeĀ® we strongly suggest testing the strain with the Insertion Sequence (IS) Detection Kit to verify that no transposable elements are present.
Kit Components
pSX2 Expression Vector, 10 Ī¼gĀ in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA
Quality Control
The vector is confirmed to be IS-free and for functionality.
Storage
Store at ā¤ -70 Ā°C. Do not store in a frost-free freezer.
Shipping and Handling
This kit is shipped on dry ice, and shipping and handling are additional. For US orders, shipping and handling is $160. For orders outside the US, there is a handling fee of $50 plus actual shipping costs.Ā Please provide a Fedex account number we may use to bill the shipping costs.
