• Facebook
  • Twitter
  • LinkedIn
  • My account
  • Register
1-888-513-7075
Scarab Genomics
  • Products
    • Vaccine Carrier Proteins
      • CRM197 protein
      • Protein D
    • Clean Genome® E. coli strains
      • Overview
      • Clean Genome®
      • Clean Genome® ΔrecA
      • Clean Genome® LowMut
      • Clean Genome® LowMut ΔrecA
      • Clean Genome® LowMut ΔrecA Blue
      • Clean Genome® LowMut T7
    • Vectors
      • pSX2 Expression Vector
    • IS Detection
      • Insertion Sequence Detection Kit
  • Licensing
  • Technology
    • Clean Genome® E. coli
    • C-Flow™ Continuous Fermentation
    • References
  • Contact & support
    • News
    • FAQs
    • About us
  • Search
  • Menu Menu
  • 0Shopping Cart
CRM197 mutant diphtheria toxin crystal structure 4AE1 as visualized using DNASTAR's Lasergene Protean 3D

CRM197 protein

$225.00 – $2,000.00

Clear

SKU: CRM197 Categories: CRM197, Vaccine Carrier Proteins
  • Description

Description

Cross-Reactive Material 197 (CRM197) is a non-toxic mutant form of the 58-kd diphtheria toxin that serves as a carrier protein for polysaccharides and haptens in a number of conjugate vaccines, to enhance their immunogenicity.

The effectiveness of this approach has been shown with Prevnar®, Pfizer’s Pneumococcal conjugate vaccine, which contains CRM197 conjugated to weakly antigenic sugars to render the vaccine more immunogenic while maintaining disease specificity.

CRM197 is the product of a single nucleotide change in the diphtheria toxin gene to create a change from glutamic acid to glycine at position 52 of the protein, disabling the ADP-ribosylation activity of the A chain, thereby attenuating toxicity (Giannini et al., Nucl. Acids Res. 12:4063, 1984; Mekada & Uchida, J. Biol. Chem. 260:12148,1985).

At Scarab we produce CRM197 efficiently using a recombinant Clean Genome® E.coli host in our C-FLOW™ continuous fermentation system. This stable and robust process consistently produces CRM197 proteins that meet strict quality specifications, ensuring routine lot-to-lot consistency.

 

Scarab’s research-grade CRM197 exhibits greater purity than those tested from other vendors by SDS-PAGE.

Overcoming non-disease antigen specific immunogenicity

There is evidence that CRM197-conjugate vaccines elicit an immune response to CRM197 as well as to the disease antigen. Such immune responses could then impair the effectiveness of subsequent injections of vaccines for the same or other diseases if these vaccines use the CRM197 conjugate. Therefore, future conjugate vaccines may need to deploy alternative protein conjugates. This is why Scarab Genomics also offers Protein D, which has been successfully used as a conjugate for example in GSK’s Synflorix® vaccine.

CRM197 is for Research Use Only, Not for use in humans or as a diagnostic agent.

Specifications

CAS Number 92092-36-9

  • 4 mg/mL in 150 mM NaCl, 25 mM HEPES, pH 7.4 via absorbance at 280 nm using an E0.1 % = 1.07 for a 1 mg/mL solution. 0.2 μm-filtered frozen solution, store at -70 °C.
  • > 95 % pure by SDS-PAGE
  • Endotoxin < 25 EU/mg
  • Conjugation capacity confirmed by a quantitative functional assay
  • Liquid formulation stable for >= 3 years at ??? °C
  • Immunogenicity confirmed by animal studies
  • Free of Methylation, Acetylation, Gluconoylation
  • A and B subunits < 5 %
  • Dimer < 5 %
  • Biosimilar to native CRM197 from Corynebacterium diphtheriae – amino acid sequence, mass spectrometry and circular dichroism analyses confirm the bioequivalence of Scarab’s CRM197 made in Clean Genome® E. coli.

Handling

  • To minimize aggregation, thaw CRM197 in a 37 °C water bath for 1 hour.
  • Repeated freeze-thaw cycles can degrade material. To minimize freeze-thaw cycles, aliquot CRM197 to match desired use.
  • Handle product gently, DO NOT VORTEX.

 

Image credit: CRM197 mutant diphtheria toxin crystal structure 4AE1 as visualized using DNASTAR’s Lasergene Protean 3D

Related products

  • Haemophilus influenzae Protein D structure predicted using DNASTAR's NovaFold application, visualized in Lasergene Protean 3D

    Protein D

    $225.00 – $2,000.00
    Select options
© Scarab Genomics, LLC
All rights reserved
  • About us
  • News
  • Shipping
  • Licensing
  • FAQs
  • Contact & support
  • Limited Use License
  • Terms and Conditions
  • Privacy Policy
  • Trademarks
Scarab Genomics, LLC
1202 Ann Street
Madison, WI 53713 USA
Website by FisherPaul
pSX2 Expression Vector Haemophilus influenzae Protein D structure predicted using DNASTAR's NovaFold application, visualized in Lasergene Protean 3D Protein D
Scroll to top

This site uses cookies. By continuing to browse the site, you are agreeing to our use of cookies.

Continue


How we use cookies

We may request cookies to be set on your device. We use cookies to let us know when you visit our websites, how you interact with us, to enrich your user experience, and to customize your relationship with our website.

Click on the different category headings to find out more. You can also change some of your preferences. Note that blocking some types of cookies may impact your experience on our websites and the services we are able to offer.

Essential Website Cookies

These cookies are strictly necessary to provide you with services available through our website and to use some of its features.

Because these cookies are strictly necessary to deliver the website, refuseing them will have impact how our site functions. You always can block or delete cookies by changing your browser settings and force blocking all cookies on this website. But this will always prompt you to accept/refuse cookies when revisiting our site.

We fully respect if you want to refuse cookies but to avoid asking you again and again kindly allow us to store a cookie for that. You are free to opt out any time or opt in for other cookies to get a better experience. If you refuse cookies we will remove all set cookies in our domain.

We provide you with a list of stored cookies on your computer in our domain so you can check what we stored. Due to security reasons we are not able to show or modify cookies from other domains. You can check these in your browser security settings.

Google Analytics Cookies

These cookies collect information that is used either in aggregate form to help us understand how our website is being used or how effective our marketing campaigns are, or to help us customize our website and application for you in order to enhance your experience.

If you do not want that we track your visit to our site you can disable tracking in your browser here:

Other external services

We also use different external services like Google Webfonts, Google Maps, and external Video providers. Since these providers may collect personal data like your IP address we allow you to block them here. Please be aware that this might heavily reduce the functionality and appearance of our site. Changes will take effect once you reload the page.

Google Webfont Settings:

Google Map Settings:

Google reCaptcha Settings:

Vimeo and Youtube video embeds:

Other cookies

The following cookies are also needed - You can choose if you want to allow them:

Privacy Policy

You can read about our cookies and privacy settings in detail on our Privacy Policy Page.

Privacy Policy