Protein Expression
Protein Expression
Cloning
Chemically Competent Cells
Cloning
Secondary Structure
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van der Heijden et al. reported 12.85% of an HPV pDNA vaccine plasmid contained an IS2 although none had been detected in their master cell bank. They calculated that “it would have to be at a frequency below 1 in 10,000” to be undetectable. This study shows that massive expansion of IS elements from a very low starting level can be catastrophic. The authors conclude that the only way to avoid the problem is to use an IS-free host. Learn More

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